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KMID : 0381920000300040413
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Korean Journal of Microscopy
2000 Volume.30 No. 4 p.413 ~ p.421
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Thermosome-like Protein from Hyperthermophilic Archaeon Thermococcus Profundus; Purification and Structural Analysis
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Kim Suk-Kyoung
Lee Mi-Hong
Park Seong-Cheol
Cheong Gang-Won
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Abstract
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We have isolated a large cylindrical protein complex from hyperthermophile archaeon Thermococcus profundus. Structural analysis by image processing of electron micrographs suggests that the complex is composed of two stacked rings of eight subunits each; the ring enclose a central channel. The purified protein was shown to be a homomultimer of 60 kDa subunit (P60 complex). It exhibits an extremely thermostable ATPase activity with a temperature optimum of $80^{\circ}C$. This protein complex may play an important role in the adaptation of thermophile archaeon to life at high temperature.
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KEYWORD
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Electron microscopy, Heat stable, Hyperthermophile archaeon, Image processing, Thermosome
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